Colorectal cancer (CRC) is the third most common cancer and the fourth most common cause of cancer-related death, with its incidence increasing year by year.The heat-shock protein 70 (Hsp70) is important effectors to protect the intracellular proteins from misfolding or aggregation, also inhibit cell death and signaling cascades that preserve the intracellular signaling pathways which are critical for cell survival. The expression of Hsp70 is frequently abnormally high in cancer cells, they participate in oncogenesis and in resistance to chemotherapy; therefore, the inhibition of Hsp70 has become an interesting strategy in cancer therapy. The aim of the study was to perform histopathological examination and immunohistochmical analysis of paraffin-embedded sections prepared from biopsies taken from colorectal lesions of patients diagnosed with different stages of CRC to show expression of Hsp70 in different stages. Methods: The study involved (146) men and women who were clinically diagnosed with CRC by the general surgery doctor .The patient age range between 36 and 80 years .This study designs as prospective study , all samples were taken from patients in multicenter during the period from December 2021 to December 2022 . Histopathological and immunohistochemical analysis of tissues were performed for 52 patients only. Results: the results showed that Hsp70 expression with CRC moderately differentiated (grade I1) was 59.6% in total staining reaction, followed by the CRC poorly differentiated (grade III) 24.9% , while CRC (grade I) the expressions of (Hsp70) have been reported (7.69% ) respectively. According to the staging of CRC, the study included 5 patients with stage I, (15) patients with stage II, 23 patients with stage III and 9 patients with stage IV. The reaction for Hsp70 expression in (stage I) was weak ( 7.69% ) ,while in( stage II) the weak reaction was (5.7 %),the moderate was ( 17.3% ) , while in the stage III the weak reaction was (3.84) , and the moderate was( 25% ) and strong reaction was (15.3%),while in stage IV the moderate was ( 11.53% ) and strong reaction was (5.7%). Conclusions: The biomarker is aberrantly overexpressed in CRC and high expression of Hsp70 biomarker induced by associated with high grades of CRC , in contrast the low expression level associated with low grade of CRC.