In the face of escalating antimicrobial resistance (AMR), Antimicrobial Susceptibility Testing (AST) has emerged as a crucial tool in managing infectious diseases and curbing the spread of resistant pathogens. This review article delves into the disk diffusion methods, notably the Kirby-Bauer and Stokes techniques, underscoring their pivotal role in guiding antimicrobial therapy and contributing to epidemiological surveillance. While highlighting the operational principles, procedural nuances, and interpretative frameworks of these methods, the review also addresses the inherent challenges they face, including limitations with slow-growing bacteria and potential interpretative inaccuracies. Amidst these challenges, recent technological advancements promise significant improvements in the accuracy, reliability, and efficiency of AST. Innovations such as automated zone reading, enhanced media, and real-time monitoring, coupled with the potential integration of artificial intelligence and genomic data, are poised to revolutionize AST. This article underscores the imperative for ongoing innovation and adaptation in AST methodologies to effectively combat the evolving threat of AMR, emphasizing a collaborative global effort towards this end. As we navigate the complexities of antimicrobial resistance, the advancements in disk diffusion methods and the prospect of integrating cutting-edge technologies herald a new era of precision in the management of infectious diseases, promising to enhance patient outcomes and safeguard public health.