Introduction: Organ donation is one of the greatest medical marvels of the twentieth century which has saved the lives of several patients. The disparity between the huge demands for the organs and their poor supply is the main issue. The total organ donation shortage in our country can be met even if only few victims involved in fatal accidents serve as organ donors. Objectives: To assess the existing knowledge regarding organ donation among students, to determine the effectiveness of structured teaching program on knowledge regarding organ donation among students, to find out association between post-test knowledge scores with their selected demographic variables. Methodology: A pre-experimental study was conducted among students in Government Degree College Sanjauli, Shimla (H.P.). Sample of 60 students were selected using convenient sampling technique. Data was collected using self-structured questionnaire related to organ donation. The gathered data was analyzed by using descriptive and inferential statistics. Results: The study findings revealed that 42 (70%) had poor knowledge, 18 (30%) had fair knowledge and none of them had good knowledge in pre-test. In post-test 2 (3.3%) had poor knowledge, 49 (81.7%) had fair knowledge and 9 (15%) had good knowledge. The study findings also showed that there was a significant improvement in the post-test. The mean score of knowledge in pre-test was 17.30 and in post-test was 27.53 with‘t’ value 16.210 which was significant at ‘p’ <0.001. Conclusion: The study concluded that structured teaching program was effective in improving knowledge regarding organ donation among students.